12 0112 82 us cd11c hamster anti mouse cd11c  (Bio-Rad)

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy

doi: 10.1681/ASN.2019080827

Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining

Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310) R&D Systems, MAB1757 (US) Lipocalin-2 (NGAL) (for double staining) Rabbit-anti-NGAL (polyclonal) Thermo Fisher Scientific, PA5–79590 (US) Cytokeratin (Pan CK) Mouse-anti-pan Cytokeratin (clone Lu5) Abcam, ab17155 (UK) Proliferation (Ki-67, MIB1) Mouse-anti-human (clone KI-67P) Dianova, DIA-505 (GER) Proliferation (PCNA) Mouse-anti-PCNA (clone PC10) Calbiochem/Merck, NA03 (GER) Sm22 α /Transgelin Rabbit-anti-Transgelin (polyclonal) Abcam, ab14106 (UK) Tamm–Horsfall protein Rabbit-anti-human THP (polyclonal) SantaCruz, sc-20631 (GER) TNFR1 Rabbit-anti-TNFR1 (polyclonal) Abcam, ab19139 (UK) TNFR2 Rabbit-anti-TNFR2 (clone EPR1653) Abcam, ab15116 (UK) Vimentin Mouse-anti-vimentin (clone V9) Dako/Agilent, M0725 (US) Open in a separate window α -SMA, α –skeletal muscle actin; AQ-2, aquaporin-2; CD, cluster of differentiation; GER, Germany; CHE, Switzerland; F4/80, EGF-like module–containing mucin-like hormone receptor–like 1; TIM-1, T-cell immunoglobulin and mucin domain 1; HAVCR, Hepatitis A virus cellular receptor; NGAL, neutrophil gelatinase–associated lipocalin, lipocalin-2; CK, cytokeratin; PCNA, proliferating cell nuclear antigen; SM22 α , Smooth muscle protein 22- α /Transgelin; THP, Tamm–Horsfall protein, uromodulin; TNFR, tumor necrosis factor receptor.

Techniques: Immunohistochemistry, Immunofluorescence, Double Staining